DESCRIPTION MW= 36.8 kDa. 746-1060 domain of nsp3 (papain-like proteinase, nsp3d, Plpro) from SARS-CoV-2, expressed in E. coli. The protein consists in residues 746-1060 (NCBI reference sequence YP_009725299.1) with an additional C-term his-tag (highlighted in italic in the scheme below).
SEQUENCE Numeration of the nsp3 from NCBI YP_009725299.1
750 760 770 780 790 800 M-EVRTI KVFTTVDNIN LHTQVVDMSM TYGQQFGPTY LDGADVTKIK PHNSHEGKTF 810 820 830 840 850 860 YVLPNDDTLR VEAFEYYHTT DPSFLGRYMS ALNHTKKWKY PQVNGLTSIK WADNNCYLAT 870 880 890 900 910 920 ALLTLQQIEL KFNPPALQDA YYRARAGEAA NFCALILAYC NKTVGELGDV RETMSYLFQH 930 940 950 960 970 980 ANLDSCKRVL NVVCKTCGQQ QTTLKGVEAV MYMGTLSYEQ FKKGVQIPCT CGKQATKYLV 990 1000 1010 1020 1030 1040 QQESPFVMMS APPAQYELKH GTFTCASEYT GNYQCGHYKH ITSKETLYCI DGALLTKSSE 1050 1060 YKGPITDVFY KENSYTTTIK LEHHHHHH
ISOTOPIC ENRICHMENT none
SUPPLIED AS Protein solution. The concentration is calculated from the absorbance at 280nm (ε280 = 45895 M-1cm-1).
STORAGE -20°C. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.
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